This biochemical assay determines the power of a microorganism to supply the enzyme lysine decarboxylase. The enzyme removes the carboxyl group from the amino acid lysine, producing cadaverine and carbon dioxide. A optimistic result’s sometimes indicated by a shade change within the development medium from yellow to purple, reflecting an alkaline pH shift attributable to the buildup of cadaverine. A adverse result’s indicated by a yellow shade, that means no decarboxylation has occurred.
Differentiating bacterial species is essential for correct identification in medical, analysis, and industrial settings. This particular assay offers useful data for distinguishing enterobacteria and different gram-negative bacilli, notably in eventualities the place phenotypic traits overlap. Its historic significance lies in providing a comparatively easy and cost-effective technique for microbial classification, contributing to a deeper understanding of bacterial metabolism and variety.
